I already have the data just you need to complete the questions and the follow the experiment step by step to answer what should required for each question.

BIOL337 Module 4 Worksheet (50 pts.)

Name:

Section 1: HWE and TAS2R38

1. Record the class genotype frequencies and calculate the observed allele frequencies

for the class data. 3pts

Homozygous

Taster (TT; p2)

2.

Frequency

of t (q)

Calculate the expected genotype frequencies and the expected number of students with

each genotype using the observed allele frequencies from above. 3pts

Homozygous taster

TT (p2)

Exp.

Exp. Num.

Frequency

Students

3.

Observed Data

Homozygous Non- Frequency

taster (tt; q2)

of T (p)

Heterozygous

Taster (Tt; 2pq)

Expected Data

Heterozygous taster

Tt (2pq

Exp.

Exp. Num.

Frequency

Students

Homozygous nontaster

tt (q2)

Exp.

Exp. Num.

Frequency

Students

Perform a chi-squared test to determine with 95% certainty whether our TAS2R38

data are in HWE and explain your results in the space below. 5pts

Note: Although we typically consider the degrees of freedom to be the number of categories minus one,

in this case the three categories are derived from only two terms, p and q, so for this test use df=1.

Think of it this way, if we know p then we can calculate q and p2 and 2pq and q2, so there is only one

variable free to vary (p in this example) and therefore only one degree of freedom.

(Obs.-Exp.)2

Exp.

Genotype

Obs. Exp.

TT

Tt

tt

Totals

2 =

4.

If you observed deviations from HWE, why might our class data show departures

from HWE expectations? If you did not observe deviations, what does that mean about

our population? Be specific. 5pts.

Section 2 Part 1: Polygenes for height (coin data)

5. Record your coin flipping data in the table below. 4pts

H1 alleles from father

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

H1 alleles from mother

Total doses of H1

6. Compile your “dosage” and phenotype data in the table below. 4pts

Genotype*

# H1 allele

“doses”

Height (in)

Count (Fig. 4)

Frequency

(Fig. 4)

Count

(Coin)

Frequency

(Coin)

Standardized

Count

H2H2

H2H2

H2H2

H2H2

H 1H2

H 2H2

H 2H2

H 2H2

H1H1

H2H2

H2H2

H2H2

H1H1

H1H2

H2H2

H2H2

H1H1

H1H1

H2H2

H2H2

H1H1

H1H1

H1H2

H2H2

H1H1

H1H1

H1H1

H2H2

H1H1

H1H1

H1H1

H1H2

H1H1

H1H1

H1H1

H1H1

0

1

2

3

4

5

6

7

8

60-61

8

62-63

12

64-65

20

66-67

26

68-69

18

70-71

18

72-73

20

74-75

5

76-77

4

0.06

0.09

0.15

0.20

0.14

0.14

0.15

0.04

0.03

7. Compare the frequency distribution obtained from the coin flip data to the data

pictured in the Figure 4 photo. Do your simulated coin data match the student data

from the photo? Explain your findings in the space below. 7pts

Note: You cannot compare the frequencies with a chi square test. You must use

standardized count data for each dosage category.

(Obs.-Exp.)2

Exp.

Dosage Obs. Exp.

0

1

2

3

4

5

6

7

8

Totals

2 =

Section 2 Part 2: Polygenes for ridge count (fingerprint data)

8. Enter your fingerprint data in the table. For “Pattern”, enter Arch, Loop or Whorl.

4pts

Thumb

Right Hand

Third

Second

Fourth

Fifth

Pattern

Ridge Count

Ridge Count

Total

Thumb

Left Hand

Third

Second

Fourth

Fifth

Pattern

Ridge Count

Ridge Count

Total

9. Enter the class data in the tables below. 4pts

Pattern

Loop

Class Data

Prior Data

250

68.9%

Whorl

90

26.1%

Arch

20

5.0%

Total

360

100%

Note: These data are

for number of fingers

with each pattern, so

36 students X 10

fingers = 360 total obs.

Num. allele

“doses”

0

1

2

3

4

5

6

7

8

9

10

11

12

Num. Ridges

2040

4060

6080

80100

100120

120140

140160

160180

180200

200220

220240

240260

260280

Count Prior

Class

0

2

3

1

5

6

5

8

4

0

1

1

0

Frequency

0.00

0.06

0.08

0.03

0.14

0.17

0.14

0.22

0.11

0.00

0.03

0.03

0.00

Class Count

Class Freq.

Standardized

Count

10. Compare the class fingerprint pattern data to the prior class. Do our data match the

previous student data? Explain your findings and draw conclusions in the space

below. 5pts

(Obs.-Exp.)2

Exp.

Dosage Obs. Exp.

Loop

Whorl

Arch

Totals

2 =

11. Based on the conclusion you reached for the height data, compare the class ridge

count data to the prior class data and predict the outcome of a chi square test (no

need to conduct the test, just explain qualitatively whether the differences appear

large enough to reject the null). 4pts

12. Consider the total ridge count category to which you belong. What is the minimum

number of doses you could have inherited from your mother and what is the

maximum number of doses you could have inherited from your father? Explain. 2pts

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